serum il‐34 levels (R&D Systems)
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Serum Il‐34 Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serum+il%E2%80%9034+levels/pmc07439428-58-0-8?v=R%26D+Systems
Average 90 stars, based on 1 article reviews
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1) Product Images from "IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer"
Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer
Journal: Journal of Clinical Laboratory Analysis
doi: 10.1002/jcla.23335
Figure Legend Snippet: Protein expression of IL‐34 from human PTC samples. A, Western blot analysis of IL‐34 expression in PTC tissues, their adjacent noncancerous tissues, and normal controls. B, The ratio of IL‐34/GAPDH was determined to give a mean net density. C, Real‐time PCR analysis of IL‐34 expression in PTC tissues, their adjacent noncancerous tissues, and normal controls. Data were presented as the means ± standard error. ** P < .01 was considered significant
Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction
Figure Legend Snippet: IL‐34 promotes proliferation on PTC cells. Cell proliferation was determined via the CCK‐8 assay to examine the proliferation of TPC‐1 (A) and K1 cells (B) in accordance to transfected with si‐NC or si‐IL‐34. Cell proliferation in TPC‐1 (C) and K1 cells (D) which were infected with lenti‐vector or lenti‐IL‐34. Data were presented as the means ± standard error. ** P < .01 was considered significant
Techniques Used: CCK-8 Assay, Transfection, Infection, Plasmid Preparation
Figure Legend Snippet: IL‐34 inhibits apoptotic rates on PTC cells. Cell apoptosis was determined by flow cytometry after transfected with si‐IL‐34 or si‐NC in TPC‐1 (A) and K1 cells (B). Cell apoptosis in TPC‐1 (C) and K1 cells (D) which were infected with lenti‐vector or lenti‐IL‐34. The apoptotic rates were shown. Data were presented as the means ± standard error. ** P < .01 was considered significant
Techniques Used: Flow Cytometry, Transfection, Infection, Plasmid Preparation
Figure Legend Snippet: IL‐34 promotes the invasion via on PTC cells. Two PTC cell lines were not only transfected with si‐IL‐34 or si‐NC were also infected with lenti‐vector or lenti‐IL‐34 for 48 h. A, The protein expression levels of E‐cadherin, N‐cadherin, and Vimentin were measured by Western blotting in PTC cells. The ratios of EMT biomarkers when transfected with si‐IL‐34 or si‐NC were determined to give a mean net density in TPC‐1 (B) and K1 cells (C). The ratios of EMT biomarkers when infected with lenti‐vector or lenti‐IL‐34 were determined to give a mean net density in TPC‐1 (D) and K1 cells (E). Data were presented as the means ± standard error. ** P < .01 was considered significant
Techniques Used: Transfection, Infection, Plasmid Preparation, Expressing, Western Blot
Figure Legend Snippet: IL‐34 promotes the invasion and activates the ERK signaling pathway on PTC cells. Two PTC cell lines were transfected with si‐IL‐34 or si‐NC were also infected with lenti‐vector or lenti‐IL‐34 for 48 h. A, The protein expression levels of p‐ERK and ERK were measured by Western blotting in PTC cells. The ratios of ERK and p‐ERK when transfected with si‐IL‐34 or si‐NC were determined to give a mean net density in TPC‐1 (B) and K1 cells (C). The ratios of ERK and p‐ERK when infected with lenti‐vector or lenti‐IL‐34 were determined to give a mean net density inTPC‐1 (D) and K1 cells (E). Data were presented as the means ± standard error. ** P < .01 was considered significant
Techniques Used: Transfection, Infection, Plasmid Preparation, Expressing, Western Blot